0267 Development and validation of low-level Transcranial Electrical Stimulation to enhance slow oscillations during human NREM sleep

Abstract Introduction Sleep disruption, and in particular decreased NREM stage N3 sleep, has been implicated cognition pathogenesis of Alzheimer's Disease (AD). Several recent findings suggest that enhancing sleep may mitigate impairments due to chronic disruption. However, approaches such as transcranial electrical stimulation (TES) have limited by discomfort, awakenings, lack blinding. In response the need develop low-level TES, our team first identified source slow oscillations (SOs) using high-density EEG array modeling (Sourcerer®), which localized within anterior limbic brain structures. Next, we showed TES targeted this region induced enhanced SOs during laboratory. More recently, developed a portable device with minimum deployable set electrodes targeting SO-generating regions: Wireless Interface Sensor Pod (WISP). Methods Thirteen healthy adults (m = 42 years) participated three all-night recordings where they received low level (0.5 mA) designed target regions sham (placebo). was initiated after 4 minutes naturally occurring N2 included five blocks each separated one minute rest periods. Subjects were blinded conditions, outcomes feasibility, acceptability, total time stage. A convolutional neural network (CNN) developed, trained, tested on manually scored staging for automated scoring. Results rated WISP highly feasible acceptable. adequately versus stimulation. When compared session, limbic-targeted significantly increased duration spectral power 0.5-1 Hz frequency band. Finally, CNN able detect stages good accuracy (84%, kappa 0.77). Conclusion These results electrodes, when specifically sites, is feasible, acceptable, maintains blinding, increases deep (N3) sleep. The promising method both assess manipulate home setting. Support (if any) DoD MOMRP MTEC-21-06-MPAI-129